Enzymes of Interest
From Fungal Genomics
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Families of Interest
- glycoside hydrolases
- endo-acting cellulases
- exo-acting glucosidases
- endo-glucanases
- ligninases
- lignin peroxidases
- laccases - copper-containing oxidase enzymes
- pectinases and pectinesterases
- pectin lyases
- rhamnogalacturonan lyase
- pectin methylesterase
- polygalacturonase
- hemicellulase enzymes
- endo-1,4-beta-xylanases
- feruloyl esterase
- α-glucuronidases
- arabinofuranosidases
- acetylxylan esterases
- oxidoreductases
- cellobiose dehydrogenase
- Manganese peroxidase
- versatile peroxidase
Enzyme Substrates
- Cellulose - a polysaccharide consisting of a linear chain of several hundred to over ten thousand β(1→4) linked D-glucose units. Cellulose is the structural component of the primary cell wall of green plants (~33% of all plant matter).
- Lignin - a cross-linked racemic macromolecule with molecular masses in excess of 10,000 u. It is relatively hydrophobic and aromatic in nature. There are three monolignol monomers, methoxylated to various degrees: p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol. Lignin is an integral part of the secondary cell walls of plants and constitutes from a quarter to a third of the dry mass of wood.
- Pectin - a family of complex polysaccharides that contain 1,4-linked α-D-galactosyluronic acid residues. Pectins are present in most primary cell walls and particularly abundant in the non-woody parts of terrestrial plants.
- Hemicellulose - can be any of several heteropolymers (matrix polysaccharides) with a random, amorphous structure. It consists of 500-3000 sugar units (including xylose, mannose, galactose, rhamnose, and arabinose) with a branched structure. Hemicellulose is present in almost all plant cell walls.
- Cellobiose - is a disaccharide of two glucose molecules linked in a β(1→4) bond. It can be obtained by enzymatic or acidic hydrolysis of cellulose and cellulose rich materials such as cotton, jute, or paper.