EXG17B_YEAST

Name and origin

Gene Name

Name:	exg17B
Other given name:	BGL2

Protein names

Common name:	exo-1,3-beta-glucanase
EC systematic name:	3-beta-D-glucan glucohydrolase
Other names:	3-beta-D-glucan glucohydrolase; beta-1,3-glucan exo-hydrolase; exo (1-3)-glucanohydrolase; 1,3-beta-glucan glucanohydrolase

Organism

Species:	Saccharomyces cerevisiae
Strain:	X2180-1A
Taxonomic identifier	4932
Taxonomic lineage	Eukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Saccharomyces

Enzyme activity

Catalyzes the hydrolysis of beta-D-glucose residues from the non-reducing ends of beta-1,3-linked glucans to release alpha-glucose. This enzyme works rapidly on oligosaccharides but very slowly on laminaribiose.

Biochemical Properties

General Properties

Expression host
Substrate
Assay
Temperature Optimum (°C)
Temperature Stability (°C)
pH Optimum
pH Stability

Kinetic Properties

Activity Assays

Host	Substrate	Conditions	Assay Name	Specific Activity	Relative Activity %
native	laminarin	50mM sodium acetate buffer, pH 5.5, 30°C	Product Analysis	active

Product Analysis

Host	Substrate	Conditions	Assay Name	Product Formed
native	laminarin	50mM sodium acetate buffer, pH 5.5, 30°C	thin layer chromatography

Kinetic Assays

Host	Substrate	Conditions	Assay Name	km	kcat(s-1)	Vmax

Other Features

Strongly binds to chitin and beta-1,3-glucan in cell walls.

Enzyme Annotation

Enzyme Commission	3.2.1.58	3-beta-D-glucan glucohydrolase

Gene Ontology

Go number	Go term	Evidence code

Biological process

GO:0006076

(1->3)-beta-D-glucan catabolic process

IDA

Molecular Function

GO:0004338

glucan exo-1,3-beta-glucosidase activity

IDA

Cellular Component

GO:0005618

cell wall

IDA

Literature

Molecular cloning of a cell wall exo-beta-1,3-glucanase from Saccharomyces cerevisiae.

Authors	Klebl F, Tanner W
Journal	Journal of bacteriology
Address	Lehrstuhl fur Zellbiologie und Pflanzenphysiologie, Universitat Regensburg, Federal Republic of Germany.
Abstract	A major protein of Saccharomyces cerevisiae cell walls is a 29-kilodalton glycoprotein which shows lectinlike binding to beta-1,3-glucan and chitin. It was solubilized by heating isolated cell walls at 90 degrees C and purified to homogeneity by running two high-pressure liquid chromatography columns. With the sequence information of the N terminus and seven peptides, two oligonucleotides were synthesized and the gene was cloned. Its sequence is similar to those of two plant beta-glucanases, and the protein was shown to possess beta-1,3-exoglucanase activity with laminarin as substrate. Haploid yeast cells contained one copy of the gene (BGL2). Gene disruption did not result in a phenotype.
Links	Pubmed

Protein features

Signal peptide (Predicted)	SP(23aa)
N-terminal (Experimental)	23aa
Structure PMID Reference
Sequence PMID Reference
CBM
Glycosylation	N-glycosylation (approximately 10% of total protein mass),
Other Domains
Domain Order
CAZy Family	GH17

Sequences

Length	313aa
Molecular weight in kDa (experimental)	29 (SDS-PAGE), 26 (deglycosylated with endo-beta-N-acetylglucosaminidase F; SDS-PAGE),
Molecular weight in kDa (predicted)	31.74,
Protein Sequence	P15703 `MRFSTTLATA ATALFFTASQ VSAIGELAFN LGVKNNDGTC KSTSDYETEL QALKSYTSTV KVYAASDCNT LQNLGPAAEA EGFTIFVGVW PTDDSHYAAE KAALQTYLPK IKESTVAGFL VGSEALYRND LTASQLSDKI NDVRSVVADI SDSDGKSYSG KQVGTVDSWN VLVAGYNSAV IEASDFVMAN AFSYWQGQTM QNASYSFFDD IMQALQVIQS TKGSTDITFW VGETGWPTDG TNFESSYPSV DNAKQFWKEG ICSMRAWGVN VIVFEAFDED WKPNTSGTSD VEKHWGVFTS SDNLKYSLDC DFS` Get fasta
DNA Sequence	M31072 AAAATGTCAC GAAGAAAACA AGAGGATATT TGTTATTTGA TTTAACTGCC TCCTTATAAA GAAACTTTTC CTCATTCACA ATTTGGACTT CTTCTTGTTT TTTTCAATAT AAGTTTATTG AGATAGACAA CTAACCAAAA AGAAAAACGG TCAAAGATGC GTTTCTCTAC TACACTCGCT ACTGCAGCTA CTGCGCTATT TTTCACAGCC TCCCAAGTTT CAGCTATTGG TGAACTAGCC TTTAACTTGG GTGTCAAGAA CAACGATGGT ACTTGTAAGT CCACTTCCGA CTATGAAACC GAATTACAAG CTTTGAAGAG CTACACTTCC ACCGTCAAAG TTTACGCTGC CTCAGATTGT AACACTTTGC AAAACTTAGG TCCTGCTGCT GAAGCTGAGG GATTTACTAT CTTTGTCGGT GTTTGGCCAA CAGACGACAG TCATTACGCT GCTGAAAAGG CTGCTTTGCA AACCTATTTG CCAAAAATTA AAGAATCCAC TGTTGCTGGT TTCTTGGTTG GTTCTGAAGC CTTATACCGT AACGATTTGA CTGCCTCTCA ATTATCAGAC AAAATTAATG ACGTCCGTAG TGTCGTTGCT GACATTTCCG ATTCTGACGG AAAGTCATAC TCTGGTAAGC AAGTCGGTAC TGTCGATTCC TGGAATGTTT TGGTTGCTGG TTACAATTCT GCCGTTATCG AAGCTTCCGA TTTTGTTATG GCTAACGCGT TCTCCTACTG GCAAGGTCAA ACCATGCAAA ATGCCTCTTA CTCATTCTTT GATGATATTA TGCAAGCTCT ACAGGTTATC CAATCTACTA AAGGTTCTAC CGATATTACC TTCTGGGTTG GTGAGACCGG TTGGCCAACT GATGGTACCA ACTTTGAAAG TTCTTACCCA TCTGTTGACA ACGCCAAACA ATTCTGGAAA GAAGGTATCT GTTCCATGAG AGCTTGGGGT GTTAACGTTA TTGTTTTTGA AGCCTTTGAT GAAGATTGGA AGCCAAACAC CTCTGGTACC TCTGATGTCG AGAAGCACTG GGGTGTTTTC ACTTCAAGTG ACAATTTGAA ATACTCCTTG GACTGTGACT TTTCATGAAC GCTTTAAAAA TACTCTTTAA ACAAGAATGG CTTTTTCCTT CTGTTTAGGT GAGGAATAAG CAAAAAGTAA CAAAAAAAAA TGTTATCCCT TCTTTTCTTC CTTATTTAGG GCTTTATTTT ATCTTTCACT TCTTTTACGT ATTTGAAATA TCCTTC Get fasta

Cross-references

GenBank	M31072
Uniprot	P15703
Protein	AAA34648
RefSeq Protein	NP_011798
FungiDB (formerly Broad)
JGI
PDB